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Membrane fractions from Strongyloides venezuelensis in the immunodiagnosis of human strongyloidiasis.
Corral, Marcelo Andreetta; Paula, Fabiana Martins; Gottardi, Maiara; Meisel, Dirce Mary Correia Lima; Chieffi, Pedro Paulo; Gryschek, Ronaldo César Borges.
Afiliação
  • Corral MA; Instituto de Medicina Tropical de São Paulo, São Paulo, SP, Brazil.
  • Paula FM; Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, São Paulo, SP, Brazil.
  • Gottardi M; Instituto de Medicina Tropical de São Paulo, São Paulo, SP, Brazil.
  • Meisel DM; Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, São Paulo, SP, Brazil.
  • Chieffi PP; Instituto de Medicina Tropical de São Paulo, São Paulo, SP, Brazil.
  • Gryschek RC; Instituto de Medicina Tropical de São Paulo, São Paulo, SP, Brazil.
Rev Inst Med Trop Sao Paulo ; 57(1): 77-80, 2015.
Article em En | MEDLINE | ID: mdl-25651330
Strongyloides venezuelensis is a parasitic nematode of rodents frequently used to obtain heterologous antigens for the immunological diagnosis of human strongyloidiasis. The aim of this study was to evaluate membrane fractions from S. venezuelensis for human strongyloidiasis immunodiagnosis. Soluble and membrane fractions were obtained in phosphate saline (SS and SM) and Tris-HCl (TS and TM) from filariform larvae of S. venezuelensis. Ninety-two serum samples (n = 92) were obtained from 20 strongyloidiasis patients (Group I), 32 from patients with other parasitic diseases (Group II), and 40 from healthy individuals (Group III), and were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions (SS and TS) showed 90.0% sensitivity and 88.9% specificity, whereas the membrane fractions (SM and TM) showed 95.0% sensitivity and 94.4% specificity. The present results suggest the possible use of membrane fractions of S. venezuelensis as an alternative antigen for human strongyloidiasis immunodiagnosis.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Strongyloides / Estrongiloidíase / Imunoglobulina G / Anticorpos Anti-Helmínticos / Antígenos de Helmintos Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Strongyloides / Estrongiloidíase / Imunoglobulina G / Anticorpos Anti-Helmínticos / Antígenos de Helmintos Idioma: En Ano de publicação: 2015 Tipo de documento: Article