Influence of polymerase brand on microarray-based spoligotyping in low concentrations of mycobacterial DNA.
Mol Cell Probes
; 29(2): 126-8, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25656919
ABSTRACT
Spoligotyping is a widely used typing method for the Mycobacterium tuberculosis complex. Protocols and platforms can be adapted for direct use on patient samples. Serial dilutions of genomic DNA from Mycobacterium bovis BCG strain DSM45071 were spoligotyped by array hybridization using 32 different commercial PCR polymerase preparations. In samples with very low concentrations of mycobacterial DNA, commercially available PCR polymerases differed in their performance, and some yielded no, or false, identification. Direct spoligotyping from samples with very low concentrations of mycobacterial DNA thus requires careful selection of polymerase and strict standardization.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Bacteriano
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DNA Polimerase Dirigida por DNA
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Mycobacterium tuberculosis
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article