Purification, crystallization and preliminary X-ray diffraction analysis of a soluble variant of the monoglyceride lipase Yju3p from the yeast Saccharomyces cerevisiae.
Acta Crystallogr F Struct Biol Commun
; 71(Pt 2): 243-6, 2015 Feb.
Article
em En
| MEDLINE
| ID: mdl-25664804
ABSTRACT
The protein Yju3p is the orthologue of monoglyceride lipases in the yeast Saccharomyces cerevisiae. A soluble variant of this lipase termed s-Yju3p (38.3 kDa) was generated and purified to homogeneity by affinity and size-exclusion chromatography. s-Yju3p was crystallized in a vapour-diffusion setup at 293 K and a complete data set was collected to 2.4 Å resolution. The crystal form was orthorhombic (space group P212121), with unit-cell parameters a = 77.2, b = 108.6, c = 167.7 Å. The asymmetric unit contained four molecules with a solvent content of 46.4%.
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MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Difração de Raios X
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Proteínas de Saccharomyces cerevisiae
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Monoacilglicerol Lipases
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article