TGFß3 secretion by three-dimensional cultures of human dental apical papilla mesenchymal stem cells.
J Tissue Eng Regen Med
; 11(4): 1045-1056, 2017 04.
Article
em En
| MEDLINE
| ID: mdl-25690385
ABSTRACT
Mesenchymal stem cells (MSCs) can be isolated from dental tissues, such as pulp and periodontal ligament; the dental apical papilla (DAP) is a less-studied MSC source. These dental-derived MSCs are of great interest because of their potential as an accessible source for cell-based therapies and tissue-engineering (TE) approaches. Much of the interest regarding MSCs relies on the trophic-mediated repair and regenerative effects observed when they are implanted. TGFß3 is a key growth factor involved in tissue regeneration and scarless tissue repair. We hypothesized that human DAP-derived MSCs (hSCAPs) can produce and secrete TGFß3 in response to micro-environmental cues. For this, we encapsulated hSCAPs in different types of matrix and evaluated TGFß3 secretion. We found that dynamic changes of cell-matrix interactions and mechanical stress that cells sense during the transition from a monolayer culture (two-dimensional, 2D) towards a three-dimensional (3D) culture condition, rather than the different chemical composition of the scaffolds, may trigger the TGFß3 secretion, while monolayer cultures showed almost 10-fold less secretion of TGFß3. The study of these interactions is provided as a cornerstone in designing future strategies in TE and cell therapy that are more efficient and effective for repair/regeneration of damaged tissues. Copyright © 2015 John Wiley & Sons, Ltd.
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Base de dados:
MEDLINE
Assunto principal:
Papila Dentária
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Fator de Crescimento Transformador beta3
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Células-Tronco Mesenquimais
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article