Photoreceptor cells display a daily rhythm in the orphan receptor Esrrß.
Mol Vis
; 21: 173-84, 2015.
Article
em En
| MEDLINE
| ID: mdl-25737630
ABSTRACT
PURPOSE:
Nuclear orphan receptors are critical for the development and long-term survival of photoreceptor cells. In the present study, the expression of the nuclear orphan receptor Esrrß--a transcriptional regulator of energy metabolism that protects rod photoreceptors from dystrophy--was tested under daily regulation in the retina and photoreceptor cells.METHODS:
The daily transcript and protein amount profiles were recorded in preparations of the whole retina and microdissected photoreceptor cells using quantitative PCR (qPCR) and western blot analysis.RESULTS:
Esrrß displayed a daily rhythm with elevated values at night in the whole retina and enriched photoreceptor cells. Daily regulation of Esrrß mRNA depended on light input but not on melatonin, and evoked a corresponding rhythm in the Esrrß protein.CONCLUSIONS:
The data presented in this study indicate that daily regulation of Esrrß in photoreceptor cells may contribute to their adaptation to 24-h changes in metabolic demands.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Retina
/
RNA Mensageiro
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Receptores de Estrogênio
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Ritmo Circadiano
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Células Fotorreceptoras Retinianas Bastonetes
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Metabolismo Energético
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article