Gene Transfer into Pluripotent Stem Cells via Lentiviral Transduction.
Methods Mol Biol
; 1341: 67-85, 2016.
Article
em En
| MEDLINE
| ID: mdl-25762298
ABSTRACT
Recombinant lentiviral vectors are powerful tools to stably manipulate human pluripotent stem cells. They can be used to deliver ectopic genes, shRNAs, miRNAs, or any possible genetic DNA sequence into diving and nondividing cells. Here we describe a general protocol for the production of self-inactivating lentiviral vector particles and their purification to high titers by either ultracentrifugation or ultrafiltration. Next we provide a basic procedure to transduce human pluripotent stem cells and propagate clonal cell lines.
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Base de dados:
MEDLINE
Assunto principal:
Transdução Genética
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Técnicas de Transferência de Genes
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Lentivirus
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Células-Tronco Pluripotentes
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Vetores Genéticos
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article