Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis.
Biotechnol Lett
; 37(7): 1455-62, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-25801671
ABSTRACT
OBJECTIVES:
The use of endo-arabinanase from Bacillus licheniformis (ABNase) for sugarcane saccharification has been evaluated by enzyme immobilization and commercial cocktail supplement with the immobilized heterologous protein.RESULTS:
Biochemical characterization of the purified ABNase showed that the catalytic activity was strongly inhibited by 5 mM Cu(2+), Zn(2+) or Fe(3+). The optimum pH and temperature for activity were 5.5-6.5 and 35-40 °C, respectively. The enzyme stability increased 128-fold when immobilized with glyoxyl agarose, and the hydrolysis of pretreated sugar cane biomass increased by 15 % when a commercial enzyme cocktail was supplemented with immobilized ABNase.CONCLUSION:
Pectin hydrolysis by recombinant ABNase plays a role in the effective application of enzymatic cocktails for biomass saccharification.
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Base de dados:
MEDLINE
Assunto principal:
Bacillus
/
Biomassa
/
Reatores Biológicos
/
Enzimas Imobilizadas
/
Glicosídeo Hidrolases
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article