Annexin V-induced rat Leydig cell proliferation involves Ect2 via RhoA/ROCK signaling pathway.
Sci Rep
; 5: 9437, 2015 Mar 24.
Article
em En
| MEDLINE
| ID: mdl-25807302
ABSTRACT
This study investigated the effect of annexin V on the proliferation of primary rat Leydig cells and the potential mechanism. Our results showed that annexin V promoted rat Leydig cell proliferation and cell cycle progression in a dose- and time-dependent manner. Increased level of annexin V also enhanced Ect2 protein expression. However, siRNA knockdown of Ect2 attenuated annexin V-induced proliferation of rat Leydig cells. Taken together, these data suggest that increased level of annexin V induced rat Leydig cell proliferation and cell cycle progression via Ect2. Since RhoA activity was increased following Ect2 activation, we further investigated whether Ect2 was involved in annexin V-induced proliferation via the RhoA/ROCK pathway, and the results showed that annexin V increased RhoA activity too, and this effect was abolished by the knockdown of Ect2. Moreover, inhibition of the RhoA/ROCK pathway by a ROCK inhibitor, Y27632, also attenuated annexin V-induced proliferation and cell cycle progression. We thus conclude that Ect2 is involved in annexin V-induced rat Leydig cell proliferation through the RhoA/ROCK pathway.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Proto-Oncogênicas
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Proteína rhoA de Ligação ao GTP
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Quinases Associadas a rho
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Tumor de Células de Leydig
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article