Proteomics characterization of exosome cargo.
Methods
; 87: 75-82, 2015 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-25837312
ABSTRACT
Characterization of exosomal cargo is of significant interest because this cargo can provide clues to exosome biogenesis, targeting, and cellular effects and may be a source of biomarkers for disease diagnosis, prognosis and response to treatment. With recent improvements in proteomics technologies, both qualitative and quantitative characterization of exosomal proteins is possible. Here we provide a brief review of exosome proteomics studies and provide detailed protocols for global qualitative, global quantitative, and targeted quantitative analysis of exosomal proteins. In addition, we provide an example application of a standard global quantitative analysis followed by validation via a targeted quantitative analysis of urine exosome samples from human patients. Advantages and limitations of each method are discussed as well as future directions for exosome proteomics analysis.
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
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Processamento de Proteína Pós-Traducional
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Proteoma
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Proteômica
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Exossomos
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article