Immobilization of laccase in a sponge-like hydrogel for enhanced durability in enzymatic degradation of dye pollutants.
J Colloid Interface Sci
; 450: 353-360, 2015 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-25841061
ABSTRACT
A highly stable and efficient biocatalyst was fabricated by encapsulating Trametes versicolor laccase within a chitosan grafted polyacrylamide hydrogel (denoted as Lac-PAM-CTS). Scanning electron microscopy and nitrogen adsorption-desorption tests demonstrated that channels of diameter of 10-20 µm were regularly distributed throughout the sponge-like Lac-PAM-CTS. Besides, there were massive mesopores and macropores in the lamellar walls of the hydrogel. Such a network structure reduced the diffusion resistance of the hydrogel to the target substrates. The recovered activity of the obtained Lac-PAM-CTS was 40.8%. As compared to free laccase, the Lac-PAM-CTS showed enhanced thermal and chemical stability. The positive surface charge of the Lac-PAM-CTS endowed it with a pre-enrichment effect in the treatment of anionic dyes. In a continuous six-cycle batch decoloration of Malachite Green, the Lac-PAM-CTS showed much better durability than the free laccase. The results here suggest that sponge-like hydrogel is a good supporting matrix for laccase.
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MEDLINE
Assunto principal:
Corantes de Rosanilina
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Poluentes Químicos da Água
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Proteínas Fúngicas
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Hidrogéis
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Lacase
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Enzimas Imobilizadas
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Trametes
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article