Ethyl methanesulfonate induces mutations in Caenorhabditis elegans embryos at a high frequency.
Mutat Res
; 766-767: 44-8, 2014.
Article
em En
| MEDLINE
| ID: mdl-25847271
ABSTRACT
Mutagenesis protocols typically call for exposure of late-stage larvae or adults to a mutagen with the intention of inducing mutations in a robust germ line. Instead, ca. 16,000 CB665 [unc-58(e665)] one- to four-cell embryos of the nematode Caenorhabditis elegans were hand selected and exposed to ethyl methanesulfonate (EMS) for 50min. Twenty-one reversion mutants were recovered, of which 17 were intragenic suppressors of the e665 mutation. The mutation frequency was 6.5-fold higher than when CB665 adults were similarly mutagenized, which was predicted given that cell-cycle checkpoints are muted in C. elegans embryos. The mutation spectrum was similar to that obtained after standard EMS mutagenesis.
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Base de dados:
MEDLINE
Assunto principal:
Dano ao DNA
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Caenorhabditis elegans
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Metanossulfonato de Etila
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Mutagênicos
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Mutação
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article