Expression, purification, crystallization and preliminary X-ray diffraction analysis of a type II NADH:quinone oxidoreductase from the human pathogen Staphylococcus aureus.
Acta Crystallogr F Struct Biol Commun
; 71(Pt 4): 477-82, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25849513
ABSTRACT
In recent years, type II NADH dehydrogenases (NDH-IIs) have emerged as potential drug targets for a wide range of human disease causative agents. In this work, the NDH-II enzyme from the Gram-positive human pathogen Staphylococcus aureus was recombinantly expressed in Escherichia coli, purified, crystallized and a crystallographic data set was collected at a wavelength of 0.873â
Å. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 81.8, b = 86.0, c = 269.9â
Å, contained four monomers per asymmetric unit and diffracted to a resolution of 3.32â
Å. A molecular-replacement solution was obtained and model building and refinement are currently under way.
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MEDLINE
Assunto principal:
Staphylococcus aureus
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Complexos Multienzimáticos
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NADH NADPH Oxirredutases
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article