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[Expression and clinical significance of plasma microRNA-125b level in patients with oral squamous cell carcinoma].
Gu, Wen-Li; Ye, Dong-Xia; Wu, Jing-Jing.
Afiliação
  • Gu WL; Department of Clinical Laboratory, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine. Shanghai 200011;China.E-mail:guw172@gmail.com.
Shanghai Kou Qiang Yi Xue ; 24(1): 71-5, 2015 Feb.
Article em Zh | MEDLINE | ID: mdl-25858373
ABSTRACT

PURPOSE:

To explore the expression and clinical significance of plasma microRNA-125b (miR-125b) in patients with oral squamous cell carcinoma (OSCC).

METHODS:

By Stem-loop RT real-time PCR based on the SYBR Green, we investigated the expression of plasma miR-125b in 85 OSCC patients and 46 healthy controls, then evaluated its diagnostic performance as OSCC biomarker. Data analysis was performed with SPSS 17.0 software package for one-way ANOVA and receiver operating characteristic (ROC) curve.

RESULTS:

Stem-loop RT-PCR could specifically amplify miR-125b in plasma. The expression of plasma miR-125b was significantly increased in patients with OSCC compared with healthy controls (P<0.05). ROC results showed that miR-125b could differentiate OSCC from healthy controls(AUC=0.966). As fold change 3.46 was chosen for optimal cutoff value, the diagnostic sensitivity and specificity was 89.4% and 93.5%, respectively.

CONCLUSIONS:

Due to the satisfactory diagnostic performance, plasma miR-125b can be used as a promising biomarker in OSCC.
Assuntos
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Base de dados: MEDLINE Assunto principal: Neoplasias Bucais / Carcinoma de Células Escamosas / MicroRNAs Idioma: Zh Ano de publicação: 2015 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Neoplasias Bucais / Carcinoma de Células Escamosas / MicroRNAs Idioma: Zh Ano de publicação: 2015 Tipo de documento: Article