An automated method for efficient, accurate and reproducible construction of RNA-seq libraries.
BMC Res Notes
; 8: 124, 2015 Apr 03.
Article
em En
| MEDLINE
| ID: mdl-25879881
ABSTRACT
BACKGROUND:
Integration of RNA-seq expression data with knowledge on chromatin accessibility, histone modifications, DNA methylation, and transcription factor binding has been instrumental for the unveiling of cell-specific local and long-range regulatory patterns, facilitating further investigation on the underlying rules of transcription regulation at an individual and allele-specific level. However, full genome transcriptome characterization has been partially limited by the complexity and increased time-requirements of available RNA-seq library construction protocols.FINDINGS:
Use of the SX-8G IP-Star® Compact System significantly reduces the hands-on time for RNA-seq library synthesis, adenylation, and adaptor ligation providing with high quality RNA-seq libraries tailored for Illumina high-throughput next-generation sequencing. Generated data exhibits high technical reproducibility compared to data from RNA-seq libraries synthesized manually for the same samples. Obtained results are consistent regardless the researcher, day of the experiment, and experimental run.CONCLUSIONS:
Overall, the SX-8G IP-Star® Compact System proves an efficient, fast and reliable tool for the construction of next-generation RNA-seq libraries especially for trancriptome-based annotation of larger genomes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Biblioteca Gênica
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Genoma
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Sequenciamento de Nucleotídeos em Larga Escala
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Transcriptoma
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article