Structures of aspartate aminotransferases from Trypanosoma brucei, Leishmania major and Giardia lamblia.
Acta Crystallogr F Struct Biol Commun
; 71(Pt 5): 566-71, 2015 May.
Article
em En
| MEDLINE
| ID: mdl-25945710
ABSTRACT
The structures of three aspartate aminotransferases (AATs) from eukaryotic pathogens were solved within the Seattle Structural Genomics Center for Infectious Disease (SSGCID). Both the open and closed conformations of AAT were observed. Pyridoxal phosphate was bound to the active site via a Schiff base to a conserved lysine. An active-site mutant showed that Trypanosoma brucei AAT still binds pyridoxal phosphate even in the absence of the tethering lysine. The structures highlight the challenges for the structure-based design of inhibitors targeting the active site, while showing options for inhibitor design targeting the N-terminal arm.
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MEDLINE
Assunto principal:
Aspartato Aminotransferases
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Trypanosoma brucei brucei
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Giardia lamblia
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Leishmania major
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article