In vitro studies in a myelogenous leukemia cell line suggest an organized binding of geranylgeranyl diphosphate synthase inhibitors.
Biochem Pharmacol
; 96(2): 83-92, 2015 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-25952057
ABSTRACT
A small set of isoprenoid bisphosphonates ethers has been tested in the K562 chronic myelogenous leukemia cell line to determine their impact on isoprenoid biosynthesis. Five of these compounds inhibit geranylgeranyl diphosphate synthase (GGDPS) with IC50 values below 1 µM in enzyme assays, but in cells their apparent activity is more varied. In particular, the isomeric C-geranyl-O-prenyl and C-prenyl-O-geranyl bisphosphonates are quite different in their activity with the former consistently demonstrating greater impairment of geranylgeranylation in cells but the latter showing greater impact in the enzyme assays with GGDPS. Together, these findings suggest an organized binding of these inhibitors in the two hydrophobic channels of the geranylgeranyl diphosphate synthase enzyme.
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Base de dados:
MEDLINE
Assunto principal:
Terpenos
/
Difosfonatos
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Éteres
/
Farnesiltranstransferase
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article