Association between the TCF7L2 rs12255372 (G/T) gene polymorphism and type 2 diabetes mellitus in a Cameroonian population: a pilot study.

Nanfa, Dieudonne; Sobngwi, Eugene; Atogho-Tiedeu, Barbara; Noubiap, Jean Jacques N; Donfack, Olivier Sontsa; Mofo, Edith Pascale Mato; Guewo-Fokeng, Magellan; Nguimmo Metsadjio, Aurelie; Ndonwi Ngwa, Elvis; Pokam Fosso, Priscille; Djahmeni, Eric; Djokam-Dadjeu, Rosine; Evehe, Marie-Solange; Aminkeng, Folefac; Mbacham, Wilfred F; Mbanya, Jean Claude

Nanfa, Dieudonne; Sobngwi, Eugene; Atogho-Tiedeu, Barbara; Noubiap, Jean Jacques N; Donfack, Olivier Sontsa; Mofo, Edith Pascale Mato; Guewo-Fokeng, Magellan; Nguimmo Metsadjio, Aurelie; Ndonwi Ngwa, Elvis; Pokam Fosso, Priscille; Djahmeni, Eric; Djokam-Dadjeu, Rosine; Evehe, Marie-Solange; Aminkeng, Folefac; Mbacham, Wilfred F; Mbanya, Jean Claude.

Afiliação

Nanfa D; Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
Sobngwi E; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon ; Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon ; National Obesity Center, Yaoundé Central Hos
Atogho-Tiedeu B; Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
Noubiap JJ; Department of Medicine, Groote Schuur Hospital and University of Cape Town, Cape Town, South Africa ; Medical Diagnostic Center, Yaoundé, Cameroon.
Donfack OS; Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
Mofo EP; Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
Guewo-Fokeng M; Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
Nguimmo Metsadjio A; Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.
Ndonwi Ngwa E; Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
Pokam Fosso P; Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
Djahmeni E; Department of Medicine, Groote Schuur Hospital and University of Cape Town, Cape Town, South Africa.
Djokam-Dadjeu R; Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
Evehe MS; Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
Aminkeng F; The Canadian Pharmacogenomics Network for Drug Safety (CPNDS), Center for Molecular Medicine and Therapeutics, Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
Mbacham WF; Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Public Health Research Biotechnologies, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
Mbanya JC; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon ; Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon ; National Obesity Center, Yaoundé Central Hos

Clin Transl Med ; 4: 17, 2015.

Article em En | MEDLINE | ID: mdl-25995831

ABSTRACT

ABSTRACT

BACKGROUND:

To study the relationship between the rs12255372 (G/T) polymorphism of the transcription factor 7-like 2 (TCF7L2) and type 2 diabetes mellitus (T2DM) in a Cameroonian population.

METHODS:

This case-control study included 60 T2DM patients and 60 healthy normoglycemic controls, all unrelated and of Cameroonian origin, aged above 40 years (range 40-87). The Restriction Fragment Length Polymorphism - Polymerase Chain Reaction (RFLP-PCR) was used for genotyping.

RESULTS:

The T allele frequency was significantly higher in the diabetic group (0.44) than in the control group (0.17). This allele was significantly associated to a greater risk of developing T2DM as compared to the G allele (OR = 3.92, 95% CI 2.04 - 7.67, p < 0.0001). The codominant (additive) model explained best the risk of developing the disease, as the TT genotype was significantly associated to T2DM when compared to the GG genotype (OR = 4.45, 95% CI 1.64 - 12.83, p = 0.0014). By logistic regression adjusted for age, this OR was 4.33 (95% CI 1.57 - 11.92, p = 0.005).