Effect of sodium accumulation on heterotrophic growth and polyhydroxybutyrate (PHB) production by Cupriavidus necator.
Bioresour Technol
; 191: 213-8, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-25997010
ABSTRACT
This study evaluates the effect of sodium (Na(+)) concentration on the growth and PHB production by Cupriavidus necator. Both biomass growth and PHB production were inhibited by Na(+) biomass growth became zero at 8.9 g/L Na(+) concentration while PHB production was completely stopped at 10.5 g/L Na(+). A mathematical model for pure culture heterotrophic PHB production was set up to describe the Na(+) inhibition effect. The parameters related to Na(+) inhibition were estimated based on shake flask experiments. The accumulated Na(+) showed non-linear inhibition effect on biomass growth but linear inhibition effect on PHB production kinetics. Fed-batch experiments revealed that a high accumulation of Na(+) due to a prolonged growth phase, using NaOH for pH control, decreased the subsequent PHB production. The model was validated based on independent experimental data sets, showing a good agreement between experimental data and simulation results.
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MEDLINE
Assunto principal:
Sódio
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Cupriavidus necator
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Hidroxibutiratos
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article