Non-linear optical flow cytometry using a scanned, Bessel beam light-sheet.
Sci Rep
; 5: 10751, 2015 May 29.
Article
em En
| MEDLINE
| ID: mdl-26021750
ABSTRACT
Modern flow cytometry instruments have become vital tools for high-throughput analysis of single cells. However, as issues with the cellular labeling techniques often used in flow cytometry have become more of a concern, the development of label-free modalities for cellular analysis is increasingly desired. Non-linear optical phenomena (NLO) are of growing interest for label-free analysis because of the ability to measure the intrinsic optical response of biomolecules found in cells. We demonstrate that a light-sheet consisting of a scanned Bessel beam is an optimal excitation geometry for efficiently generating NLO signals in a microfluidic environment. The balance of photon density and cross-sectional area provided by the light-sheet allowed significantly larger two-photon fluorescence intensities to be measured in a model polystyrene microparticle system compared to measurements made using other excitation focal geometries, including a relaxed Gaussian excitation beam often used in conventional flow cytometers.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fenômenos Ópticos
/
Dispositivos Lab-On-A-Chip
/
Análise de Célula Única
/
Citometria de Fluxo
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article