Effects of oestrogen deficiency on the alveolar bone of rats with experimental periodontitis.
Mol Med Rep
; 12(3): 3494-3502, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-26035209
ABSTRACT
Periodontitis is an inflammatory disease characterized by loss of connective tissue and alveolar bone, and osteoporosis is a common disease characterized by a systemic impairment of bone mass and microarchitecture. To date, the association between periodontitis and osteoporosis has remained to be fully elucidated. In the present study, an experimental rat model of periodontitis was used to explore the effects of oestrogen deficiencyinduced osteoporosis on the maxillary alveolar bone. Fortyfour female, sixmonthold SpragueDawley rats were randomly divided into four groups Control, ligature, ovariectomized (OVX), and OVX + ligature. One month after ovariectomy, rats in the ligature and OVX + ligature groups received ligatures on their first and second maxillary molars for 1 month. Fluorescent labelling was performed prior to sacrificing the animals. At the end of the experiment, the maxillae and serum were collected and subjected to microcomputed tomography analysis, confocal laserscanning microscopic observation, Van Gieson's fuchsin staining, tartrateresistant acid phosphatase staining and ELISA. Ligatures slightly reduced the alveolar bone mineral density (BMD) and bone formation rate, but significantly reduced alveolar crest height (ACH). Ovariectomy reduced the alveolar BMD, impaired the trabecular structure, reduced the bone formation rate and increased the serum levels of bone resorption markers. Animals in the OVX + ligature group exhibited a lower alveolar BMD, a poorer trabecular structure, a reduced ACH, a lower bone formation rate and higher serum levels of bone resorption markers compared with those in the control group. The results of the present study showed that ovariectomy enhanced alveolar bone loss and reduced the ACH of rats with experimental periodontitis. Thus, postmenopausal osteoporosis may influence the progression of periodontitis.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Periodontite
/
Osteoporose Pós-Menopausa
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Perda do Osso Alveolar
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Estrogênios
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article