Transient downregulation of microRNA-206 protects alkali burn injury in mouse cornea by regulating connexin 43.
Int J Clin Exp Pathol
; 8(3): 2719-27, 2015.
Article
em En
| MEDLINE
| ID: mdl-26045777
ABSTRACT
PURPOSE:
Chemical burn in cornea may cause permanent visual problem or complete blindness. In the present study, we investigated the role of microRNA 206 (miR-206) in relieving chemical burn in mouse cornea.METHOD:
An alkali burn model was established in C57BL/6 mice to induce chemical corneal injury. Within 72 hours, the transient inflammatory responses in alkali-treated corneas were measured by opacity and corneal neovascularization (CNV) levels, and the gene expression profile of miR-206 was measured by quantitative real-time PCR (qPCR). Inhibitory oligonucleotides of miR-206, miR-206-I, were intrastromally injected into alkali-burned corneas. The possible protective effects of down-regulating miR-206 were assessed by both in vivo measurements of inflammatory responses and in vitro histochemical examinations of corneal epithelium sections. The possible binding of miR-206 on its molecular target, connexin43 (Cx43), was assessed by luciferase reporter (LR) and western blot (WB) assays. Cx43 was silenced by siRNA to examine its effect on regulating miR-206 modulation in alkali-burned cornea.RESULTS:
Opacity and CNV levels, along with gene expression of miR-206, were all transiently elevated within 72 hours of alkali-burned mouse cornea. Intrastromal injection of miR-206-I into alkali-burned cornea down-regulated miR-206 and ameliorated inflammatory responses both in vivo and in vitro. LR and WB assays confirmed that Cx43 was directly targeted by miR-206 in mouse cornea. Genetic silencing of Cx43 reversed the protective effect of miR-206 down-regulation in alkali-burned cornea.CONCLUSION:
miR-206, associated with Cx43, is a novel molecular modulator in alkali burn in mouse cornea.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Hidróxido de Sódio
/
Queimaduras Químicas
/
Neovascularização da Córnea
/
Conexina 43
/
Córnea
/
MicroRNAs
/
Lesões da Córnea
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article