Sequential phosphorylation analysis using dye-tethered peptides and microfluidic isoelectric focusing electrophoresis.

Choi, Hoseok; Choi, Nakchul; Lim, Butaek; Kim, Tae-Wuk; Song, Simon; Kim, Young-Pil

Choi, Hoseok; Choi, Nakchul; Lim, Butaek; Kim, Tae-Wuk; Song, Simon; Kim, Young-Pil.

Afiliação

Choi H; Department of Life Science, Hanyang University, Seoul 133-791, Republic of Korea; Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, Republic of Korea.
Choi N; Department of Mechanical Convergence Engineering, Hanyang University, Seoul 133-791, Republic of Korea.
Lim B; Department of Life Science, Hanyang University, Seoul 133-791, Republic of Korea; Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, Republic of Korea.
Kim TW; Department of Life Science, Hanyang University, Seoul 133-791, Republic of Korea; Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, Republic of Korea.
Song S; Department of Mechanical Convergence Engineering, Hanyang University, Seoul 133-791, Republic of Korea; Institute of Nano Science and Technology, Hanyang University, Seoul 133-791, Republic of Korea. Electronic address: simonsong@hanyang.ac.kr.
Kim YP; Department of Life Science, Hanyang University, Seoul 133-791, Republic of Korea; Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, Republic of Korea; Institute of Nano Science and Technology, Hanyang University, Seoul 133-791, Republic of Korea. Electronic address: ypilkim

Biosens Bioelectron ; 73: 93-99, 2015 Nov 15.

Article em En | MEDLINE | ID: mdl-26050965

Assuntos

Técnicas Biossensoriais/instrumentação; Focalização Isoelétrica/instrumentação; Dispositivos Lab-On-A-Chip; Fosforilação; Corantes; MAP Quinases Reguladas por Sinal Extracelular/antagonistas & inibidores; MAP Quinases Reguladas por Sinal Extracelular/metabolismo; Quinase 3 da Glicogênio Sintase/antagonistas & inibidores; Quinase 3 da Glicogênio Sintase/metabolismo; Humanos; Células MCF-7; Peptídeos; Inibidores de Proteínas Quinases/farmacologia; Transdução de Sinais

Palavras-chave

Immunoprecipitation; Inhibition assay; Isoelectric focusing; Microfluidic; Protein kinase; Sequential phosphorylation

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fosforilação / Técnicas Biossensoriais / Dispositivos Lab-On-A-Chip / Focalização Isoelétrica Idioma: En Ano de publicação: 2015 Tipo de documento: Article

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