Expression, purification and crystallization of a family 55 ß-1,3-glucanase from Chaetomium thermophilum.
Acta Crystallogr F Struct Biol Commun
; 71(Pt 6): 680-3, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-26057795
ABSTRACT
A ß-1,3-glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris, purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 Å resolution were collected in-house at 293 K from a single crystal. The crystal was found to belong to space group P2(1), with unit-cell parameters a = 64.1, b = 85.8, c = 68.5 Å, ß = 93.1° and one molecule in the asymmetric unit.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Fúngicas
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Chaetomium
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Glucana 1,3-beta-Glucosidase
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article