[Determination of 2,4-dihydroxybenzophenone in mouse brain by high performance liquid chromatography].
Beijing Da Xue Xue Bao Yi Xue Ban
; 47(3): 541-7, 2015 Jun 18.
Article
em Zh
| MEDLINE
| ID: mdl-26080890
ABSTRACT
OBJECTIVE:
To optimize and establish the experimental methods for the determination of 2,4-dihydroxybenzophenone (BP-1) in mouse brain.METHODS:
BP-1 was determined by high performance liquid chromatography (HPLC) and separated by Waters Symmetry C18 (4.6 mm×250 mm, 5 µm) using isocratic elution, and the sample preparation conditions were optimized by orthogonal experiment design. The mobile phase was methanol-water (volume ratio 31) containing 3% (volume fraction) acetic acid (pH 3.40) at a flow rate of 1.0 mL/min, and ultraviolet (UV) detection wavelength was set at 290 nm. Retention time was used for qualitative analysis and internal standard method for quantitative analysis.RESULTS:
Under the optimized experimental conditions, the calibration curve was linear with a correlation coefficient of 0.999 8 over the concentration range of 0.2-10.0 mg/L. The recoveries of BP-1 were between 96.8% and 104.5%. The intra-day and inter-day precision of BP-1 were 3.5%-5.7% and 4.5%-6.4%, respectively. The extraction recoveries of BP-1 at three concentrations (0.5, 2.0, 8.0 mg/L) in the mouse brain were 90.5%, 89.5%, and 97.7%, and the matrix effect of BP-1 at these three concentrations were 102.9%, 102.7%, and 90.9%, respectively.CONCLUSION:
The method is simple, accurate, and suitable for determination of the contents of BP-1 in mouse brain.
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Base de dados:
MEDLINE
Assunto principal:
Benzofenonas
/
Química Encefálica
Idioma:
Zh
Ano de publicação:
2015
Tipo de documento:
Article