Neurogranin regulates CaM dynamics at dendritic spines.
Sci Rep
; 5: 11135, 2015 Jun 18.
Article
em En
| MEDLINE
| ID: mdl-26084473
ABSTRACT
Calmodulin (CaM) plays a key role in synaptic function and plasticity due to its ability to mediate Ca(2+) signaling. Therefore, it is essential to understand the dynamics of CaM at dendritic spines. In this study we have explored CaM dynamics using live-cell confocal microscopy and fluorescence recovery after photobleaching (FRAP) to study CaM diffusion. We find that only a small fraction of CaM in dendritic spines is immobile. Furthermore, the diffusion rate of CaM was regulated by neurogranin (Ng), a CaM-binding protein enriched at dendritic spines. Interestingly, Ng did not influence the immobile fraction of CaM at recovery plateau. We have previously shown that Ng enhances synaptic strength in a CaM-dependent manner. Taken together, these data indicate that Ng-mediated enhancement of synaptic strength is due to its ability to target, rather than sequester, CaM within dendritic spines.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Calmodulina
/
Espinhas Dendríticas
/
Neurogranina
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article