Your browser doesn't support javascript.
loading
The Cellular Proteins Grb2 and DDX3 Are Increased upon Human Cytomegalovirus Infection and Act in a Proviral Fashion.
Cavignac, Yolaine; Lieber, Diana; Laib Sampaio, Kerstin; Madlung, Johannes; Lamkemeyer, Tobias; Jahn, Gerhard; Nordheim, Alfred; Sinzger, Christian.
Afiliação
  • Cavignac Y; Institute of Medical Virology and Epidemiology of Virus Diseases, University of Tübingen, Tübingen, Germany.
  • Lieber D; Institute of Virology, University of Ulm, Ulm, Germany.
  • Laib Sampaio K; Institute of Virology, University of Ulm, Ulm, Germany.
  • Madlung J; Proteome Center, University of Tübingen, Tübingen, Germany.
  • Lamkemeyer T; Proteome Center, University of Tübingen, Tübingen, Germany.
  • Jahn G; Institute of Medical Virology and Epidemiology of Virus Diseases, University of Tübingen, Tübingen, Germany.
  • Nordheim A; Proteome Center, University of Tübingen, Tübingen, Germany.
  • Sinzger C; Institute of Virology, University of Ulm, Ulm, Germany.
PLoS One ; 10(6): e0131614, 2015.
Article em En | MEDLINE | ID: mdl-26121620
While it is well established that human cytomegalovirus (HCMV) upregulates many cellular proteins and incorporates several of them into its virion, little is known about the functional relevance of such virus-host interactions. Two cellular proteins, Grb2 and DDX3, gained our interest as they appeared enriched in virion particles and this incorporation depended on the viral tegument protein pp65, suggesting a functional relevance. We therefore tested whether the level of these proteins is altered upon HCMV infection and whether they support viral replication. Immunoblotting analyses of cellular fractions showed increased levels of both proteins in infected cells with a maximum at 2 d p.i. and a reduction of the soluble Grb2 fraction. Knockdown of either gene by transfection of siRNAs reduced viral spread not only of the cell culture adapted HCMV strain TB40/E but also of recent clinical isolates. Apparently, Grb2 and DDX3 are proviral cellular factors that are upregulated in infected cells.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por Citomegalovirus / Citomegalovirus / Proteína Adaptadora GRB2 / RNA Helicases DEAD-box Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por Citomegalovirus / Citomegalovirus / Proteína Adaptadora GRB2 / RNA Helicases DEAD-box Idioma: En Ano de publicação: 2015 Tipo de documento: Article