The role of matrix metalloproteinases and cysteine-cathepsins on the progression of dentine erosion.
Arch Oral Biol
; 60(9): 1340-5, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-26134516
ABSTRACT
OBJECTIVE:
To evaluate in vitro the effect of the inhibition of endogenous dentinal enzymes (matrix metalloproteinases-MMPs and cysteine cathepsins-CCs) on dentine erosion.DESIGN:
Dentine blocks (4mm×4mm×2mm) from sound human teeth were randomly divided into 7 groups (n=17) according to the treatment MMP- and CC-inhibitor chlorhexidine digluconate (CHX, 10mM); MMP-inhibitor galardin (G, 0.2mM); specific cathepsin B inhibitor (CCB, 0.2mM); non-specific CC inhibitor (CCE-64, 0.5µM); fluoride (F, 1.23% NaF); placebo (P) and untreated (UT). Inhibitors were applied as gels once for 1min. Specimens were submitted to 5 days of pH cycling including the erosive challenge (Coke, pH 2.64, 90s/day) and remineralisation (artificial saliva). Demineralised organic surface loss was determined profilometrically. Demineralised organic matrix (DOM) was removed with collagenase and the profile was re-evaluated in the absence of collagen fibrils. The differences in profilometric results and DOM thickness among the groups were analysed with ANOVA and Tukey's test (p<0.05).RESULTS:
Loss of demineralised tissue (µm, mean±SD) was CHX 8.4±1.7 b, G 8.6±1.9 b, CCB 9.6±1.4 a, CCE-64 9.9±1.3 a, F 9.9±1.7 a, P 10.9±2.2 a, UT 11.0±1.5 a. Loss of mineralised tissue was CHX 15.4±2.2 b, G 16.0±1.8 b, CCB 17.6±2.4 a, CCE-64 17.6±2.0 a, F 17.3±2.8 a, P 19.1±2.1 a, UT 18.9±2.4 a. MMP-inhibitors significantly reduced organic matrix and mineral loss in comparison to all the other groups (p<0.05). No statistically significant differences were found in the thickness of the remaining DOM (p=0.845).CONCLUSION:
Dentine endogenous MMPs seem to be the main enzymes responsible for DOM loss and erosion.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Fluoreto de Sódio
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Erosão Dentária
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Catepsinas
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Clorexidina
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Metaloproteinases da Matriz
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Cisteína
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Dipeptídeos
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Inibidores de Metaloproteinases de Matriz
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article