A CD44 specific peptide developed by phage display for targeting gastric cancer.
Biotechnol Lett
; 37(11): 2311-20, 2015 Nov.
Article
em En
| MEDLINE
| ID: mdl-26140900
ABSTRACT
OBJECTIVE:
To develop a peptide probe that could be used for gastric cancer detection via binding to CD44 protein with specificity and affinity.RESULTS:
A 12-mer phage peptide library was screened against immobilized CD44 protein. Bound phage counts using ELISA were performed to identify phage clones carrying the most highly selective peptide, which termed RP-1. Immunofluorescence and flow cytometry analysis indicated that the consensus peptide RP-1 could bind to CD44-positive gastric cancer cells with mean fluorescence intensities significantly higher than that of CD44-negative cells. CD44 knockdown led to decreased binding activity of RP-1 to the same cell line. Tissue array technique was used to identify the relationship (r = 0.556) between peptide binding and CD44 detection on gastric cancer tissues. Further, the hyaluronan-binding domain of CD44 was docked with RP-1 using computer modeling/docking approaches, revealing a RP-1/CD44 interaction with geometrical and energy match (-8.6 kcal/mol).CONCLUSIONS:
The RP-1 peptide we screened exhibits affinity and specificity to CD44 on cells and has the potential to be used as a candidate probe for gastric cancer cell targeting.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Neoplasias Gástricas
/
Receptores de Hialuronatos
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article