Nicotiana benthamiana cathepsin B displays distinct enzymatic features which differ from its human relative and aleurain-like protease.

Niemer, Melanie; Mehofer, Ulrich; Verdianz, Maria; Porodko, Andreas; Schähs, Philipp; Kracher, Daniel; Lenarcic, Brigita; Novinec, Marko; Mach, Lukas

Niemer, Melanie; Mehofer, Ulrich; Verdianz, Maria; Porodko, Andreas; Schähs, Philipp; Kracher, Daniel; Lenarcic, Brigita; Novinec, Marko; Mach, Lukas.

Afiliação

Niemer M; Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria.
Mehofer U; Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria.
Verdianz M; Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria.
Porodko A; Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria.
Schähs P; Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria.
Kracher D; Department of Food Science and Technology, University of Natural Resources and Life Sciences, Vienna, Austria.
Lenarcic B; Department of Chemistry and Biochemistry, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Slovenia.
Novinec M; Department of Chemistry and Biochemistry, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Slovenia.
Mach L; Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria. Electronic address: lukas.mach@boku.ac.at.

Biochimie ; 122: 119-25, 2016 Mar.

Article em En | MEDLINE | ID: mdl-26166069

Assuntos

Catepsina B/metabolismo; Cisteína Endopeptidases/metabolismo; Nicotiana/enzimologia; Peptídeo Hidrolases/metabolismo; Proteínas de Plantas/metabolismo; Sequência de Aminoácidos; Animais; Biocatálise; Western Blotting; Catepsina B/genética; Precursores Enzimáticos/metabolismo; Humanos; Concentração de Íons de Hidrogênio; Cinética; Proteínas de Plantas/genética; Proteólise; Proteínas Recombinantes/metabolismo; Células Sf9; Spodoptera; Especificidade por Substrato

Palavras-chave

Antibody; Cathepsin; Cysteine protease; Expression platform; Plant; Proteolysis

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeo Hidrolases / Proteínas de Plantas / Nicotiana / Cisteína Endopeptidases / Catepsina B Idioma: En Ano de publicação: 2016 Tipo de documento: Article

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