miRNA-221 promotes proliferation, migration and invasion by targeting TIMP2 in renal cell carcinoma.
Int J Clin Exp Pathol
; 8(5): 5224-9, 2015.
Article
em En
| MEDLINE
| ID: mdl-26191221
ABSTRACT
INTRODUCTION:
MicroRNAs (miRNAs) play important roles in tumorigenesis. In this study, we investigated the role of miR-221 in the development and progression of clear cell renal cell carcinoma (ccRCC).METHODS:
Quantitative real-time PCR (qRT-PCR) was used to measure the expression level of miR-221 in ccRCC tissues and cell lines. Then, we investigated the role of miR-221 to determine its potential roles on renal cancer cell proliferation, migration and invasion in vitro. A luciferase reporter assay was conducted to confirm the target gene of miR-221 and the results were validated in renal cancer cells.RESULTS:
In the present study, we found that miR-221 was significantly increased in ccRCC tissues and cell lines. Knocked-down expression of miR-221 remarkably inhibited cell proliferation, migration and invasion of renal cancer cells. Moreover, at the molecular level, our results suggested that TIMP2 as a direct target of miR-221 through which miR-221 promoted tumor cell proliferation, migration and invasion.CONCLUSIONS:
These findings suggested that miR-221 play an oncogenic role in the renal cancer cell proliferation, migration and invasion by directly inhibiting the tumor suppressor TIMP2, indicating miR-221 act as a potential new therapeutic target for the treatment of ccRCC.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Carcinoma de Células Renais
/
Movimento Celular
/
Inibidor Tecidual de Metaloproteinase-2
/
MicroRNAs
/
Proliferação de Células
/
Neoplasias Renais
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article