System for evaluation of oxidative stress on in-vitro-produced bovine embryos.
Reprod Biomed Online
; 31(4): 577-80, 2015 Oct.
Article
em En
| MEDLINE
| ID: mdl-26206284
ABSTRACT
This study proposed a quantitative evaluation of oxidative status (OS) in bovine embryos. Sixteen-cell stage embryos, cultured under 5% O2, were treated with oxidative stress inducer menadione (0, 1, 2.5 and 5 µmol/l) for 24 h. Blastocyst rate (BLR) was recorded and expanded blastocysts were stained with CellROX®Green (CRG; OS evaluation) and evaluated under epifluorescence microscopy (ratio of pixel/blastomere). A significant effect of menadione was observed for BLR (P = 0.0039), number of blastomeres/embryo (P < 0.0001) and OS (P < 0.001). Strong negative correlations were found between BLR and the number of blastomeres with OS evaluation, demonstrating the efficacy of this analysis to evaluate OS levels of IVF bovine embryos.
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Base de dados:
MEDLINE
Assunto principal:
Estresse Oxidativo
/
Embrião de Mamíferos
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article