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Transcription yield of fully 2'-modified RNA can be increased by the addition of thermostabilizing mutations to T7 RNA polymerase mutants.
Meyer, Adam J; Garry, Daniel J; Hall, Bradley; Byrom, Michelle M; McDonald, Hannah G; Yang, Xu; Yin, Y Whitney; Ellington, Andrew D.
Afiliação
  • Meyer AJ; Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA andy.ellington@mail.utexas.edu.
  • Garry DJ; Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.
  • Hall B; Altermune Technologies, LLC, Irvine, CA 92606, USA.
  • Byrom MM; Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.
  • McDonald HG; Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.
  • Yang X; Department of Pharmacology and Toxicology, Sealy Center for Structural Biology, University of Texas Medical Branch, Galveston, TX 77555, USA.
  • Yin YW; Department of Pharmacology and Toxicology, Sealy Center for Structural Biology, University of Texas Medical Branch, Galveston, TX 77555, USA.
  • Ellington AD; Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA Center for Systems & Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA.
Nucleic Acids Res ; 43(15): 7480-8, 2015 Sep 03.
Article em En | MEDLINE | ID: mdl-26209133
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transcrição Gênica / Proteínas Virais / RNA Polimerases Dirigidas por DNA / Mutação Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transcrição Gênica / Proteínas Virais / RNA Polimerases Dirigidas por DNA / Mutação Idioma: En Ano de publicação: 2015 Tipo de documento: Article