Development of patent Litomosoides sigmodontis infections in semi-susceptible C57BL/6 mice in the absence of adaptive immune responses.
Parasit Vectors
; 8: 396, 2015 Jul 25.
Article
em En
| MEDLINE
| ID: mdl-26209319
ABSTRACT
BACKGROUND:
One of the most advantageous research aspects of the murine model of filariasis, Litomosoides sigmodontis, is the availability of mouse strains with varying susceptibility to the nematode infection. In C57BL/6 mice, L. sigmodontis worms are largely eliminated in this strain by day 40 post-infection and never produce their offspring, microfilariae (Mf). This provides a unique opportunity to decipher potential immune pathways that are required by filariae to achieve a successful infection. In this study we tracked worm development and patency, the production of microfilariae and thus the transmission life-stage, in Rag2IL-2Rγ(-/-) mice which are deficient in T, B and NK cell populations.FINDINGS:
Although worm burden was comparable between wildtype (WT) and Rag2IL-2Rγ(-/-) mice on d30, by day 72 post-infection, parasites in Rag2IL-2Rγ(-/-) mice were still in abundance, freely motile and all mice presented high quantities of Mf both at the site of infection, the thoracic cavity (TC), and in peripheral blood. Levels of cytokine (IL-4, IL-6, TNFα) and chemokine (MIP-2, RANTES, Eotaxin) parameters were generally low in the TC of infected Rag2IL-2Rγ(-/-)mice at both time-points. The frequency of neutrophils however was higher in Rag2IL-2Rγ(-/-)mice whereas eosinophils and macrophage populations, including alternatively activated macrophages, were elevated in WT controls.CONCLUSION:
Our data highlight that adaptive immune responses prevent the development of patent L. sigmodontis infections in semi-susceptible C57BL/6 mice and suggest that induction of such responses may offer a strategy to prevent transmission of human filariasis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Citocinas
/
Imunidade Adaptativa
/
Filariose
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article