Iron Deficiency and IL1ß Polymorphisms in Helicobacter pylori-infected Children.
Helicobacter
; 21(2): 124-30, 2016 Apr.
Article
em En
| MEDLINE
| ID: mdl-26211930
ABSTRACT
BACKGROUND:
Helicobacter pylori infection has been associated with an imbalance of iron homeostasis. IL-1ß has been related with iron absorption disturbances through a variety of mechanisms. The aim of this study was to evaluate the presence of polymorphic variants for IL-1ß cluster and gastric IL1ß mRNA expression in H. pylori-infected children and their relationship with hypochlorhydria and iron deficiency (ID). PATIENTS ANDMETHODS:
Prospective study of 123 symptomatic children. At endoscopy, antral biopsies were taken for urease test, pathology and culture and blood for analysis of ferritin, transferrin, serum iron, and total iron-binding capacity. Polymorphisms in the IL-1ß cluster (positions -511, -31, +3954, ILRN) were determined by PCR-RFLP. Gastric mucosal expression of IL-1ß mRNA was determined by RT-PCR.RESULTS:
After exclusions, of 105 patients, 33 (31.4%) were H. pylori positive. Nine (8.6%) children were classified as iron deficient (ID). Helicobacter pylori positivity was associated with ID (OR 5.1; 95% CI 1.2-21.9) (p = .04). No significant differences were found in allele frequency for IL1ß gene cluster polymorphisms between infected and uninfected children. Helicobacter pylori-infected children with ID had significantly increased gastric IL1ß mRNA in comparison with infected children without ID. In addition, a significant positive correlation was observed between mucosal IL-1ß mRNA and fasting gastric juice pH. Gastric pH values were significantly increased in H. pylori-infected patients with ID compared to uninfected children.CONCLUSIONS:
The established association between H. pylori infection and ID in children may be mediated by increased gastric mucosal IL-1ß.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Polimorfismo Genético
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Infecções por Helicobacter
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Perfilação da Expressão Gênica
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Interleucina-1beta
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Deficiências de Ferro
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article