HPLC Determination of Bioactive Flavonoids in Hovenia dulcis Fruit Extracts.
J Chromatogr Sci
; 54(2): 130-5, 2016 Feb.
Article
em En
| MEDLINE
| ID: mdl-26240190
ABSTRACT
A simple, accurate and reproducible reversed-phase liquid chromatographic method was developed for qualitative and quantitative determination of four bioactive flavonoids (ampelopsin, taxifolin, myricetin and quercetin) from the fruit-stalk extract of Hovenia dulcis Thunb. Chromatographic separation was performed on a C18 column (4.6 × 150 mm, 3.5 µm) with mobile phase consisting of 0.1% acetic acid and 100% acetonitrile at a flow rate of 1.0 mL/min. The analysis was performed using a diode array detector at 365 nm. The method was validated in terms of selectivity, linearity, accuracy, precision and recovery. Good linearity was observed over the investigated concentration range (10-500 µg/mL), with correlation coefficient values greater than 0.99. The intra- and inter-day precisions over the concentration range were <3.91% (relative standard deviation), and the accuracy was between 91.57 and 106.66%. The mean recovery for all the analytes was 100.87%. This method was successfully applied in the quality assessment of bioactive flavonoids in the fruit-stalk extract of H. dulcis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Flavonoides
/
Extratos Vegetais
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Cromatografia Líquida de Alta Pressão
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Rosales
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article