MicroRNA205 promotes the tumorigenesis of nasopharyngeal carcinoma through targeting tumor protein p53-inducible nuclear protein 1.
Mol Med Rep
; 12(4): 5715-22, 2015 Oct.
Article
em En
| MEDLINE
| ID: mdl-26252115
ABSTRACT
Nasopharyngeal carcinoma (NPC) is a common type of cancer in southern China, miRNAs have been shown to be involved in the tumorigenesis of multiple cancer types. The present study aimed to explore the potential role of miR205 in NPC. Reverse transcription quantitative polymerase chain reaction was used to determine the expression levels of miR205 in 20 fresh NPC specimens and 20 normal nasopharyngeal tissues. The function of miR205 in the proliferation, migration, invasion and apoptosis of NPCderived cells was detected by MTT assay, colony formation assay, wound healing assay, Transwell assay and flow cytometry. Furthermore, a target gene of miR205 was identified using the luciferase reporter assay. The expression of miR205 was increased in NPC tissues compared with that in normal tissues. Overexpression of miR205 was found to promote the proliferation, migration and invasion of NPCderived cells, while apoptosis was suppressed. Tumor protein p53-inducible nuclear protein 1 was identified as a target gene of miR205. Overall, the present study demonstrated that miR205 may function as an oncogene in NPC tumorigenesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte
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Regulação Neoplásica da Expressão Gênica
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Neoplasias Nasofaríngeas
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MicroRNAs
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Carcinogênese
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Proteínas de Choque Térmico
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article