MicroRNA­205 promotes the tumorigenesis of nasopharyngeal carcinoma through targeting tumor protein p53-inducible nuclear protein 1.

ABSTRACT

Nasopharyngeal carcinoma (NPC) is a common type of cancer in southern China, miRNAs have been shown to be involved in the tumorigenesis of multiple cancer types. The present study aimed to explore the potential role of miR­205 in NPC. Reverse transcription quantitative polymerase chain reaction was used to determine the expression levels of miR­205 in 20 fresh NPC specimens and 20 normal nasopharyngeal tissues. The function of miR­205 in the proliferation, migration, invasion and apoptosis of NPC­derived cells was detected by MTT assay, colony formation assay, wound healing assay, Transwell assay and flow cytometry. Furthermore, a target gene of miR­205 was identified using the luciferase reporter assay. The expression of miR­205 was increased in NPC tissues compared with that in normal tissues. Overexpression of miR­205 was found to promote the proliferation, migration and invasion of NPC­derived cells, while apoptosis was suppressed. Tumor protein p53-inducible nuclear protein 1 was identified as a target gene of miR­205. Overall, the present study demonstrated that miR­205 may function as an oncogene in NPC tumorigenesis.