A DEMETER-like DNA demethylase governs tomato fruit ripening.
Proc Natl Acad Sci U S A
; 112(34): 10804-9, 2015 Aug 25.
Article
em En
| MEDLINE
| ID: mdl-26261318
ABSTRACT
In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DMLs). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only a few studies demonstrate the developmental roles of active DNA demethylation conclusively in this plant. Here, we show a direct cause and effect relationship between active DNA demethylation mainly mediated by the tomato DML, SlDML2, and fruit ripening- an important developmental process unique to plants. RNAi SlDML2 knockdown results in ripening inhibition via hypermethylation and repression of the expression of genes encoding ripening transcription factors and rate-limiting enzymes of key biochemical processes such as carotenoid synthesis. Our data demonstrate that active DNA demethylation is central to the control of ripening in tomato.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
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Solanum lycopersicum
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Metilação de DNA
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DNA Glicosilases
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Frutas
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article