Longitudinal analysis of Plasmodium sporozoite motility in the dermis reveals component of blood vessel recognition.
Elife
; 42015 08 13.
Article
em En
| MEDLINE
| ID: mdl-26271010
ABSTRACT
Malaria infection starts with injection of Plasmodium sporozoites by an Anopheles mosquito into the skin of the mammalian host. How sporozoites locate and enter a blood vessel is a critical, but poorly understood process. In this study, we examine sporozoite motility and their interaction with dermal blood vessels, using intravital microscopy in mice. Our data suggest that sporozoites exhibit two types of motility in regions far from blood vessels, they exhibit 'avascular motility', defined by high speed and less confinement, while in the vicinity of blood vessels their motility is more constrained. We find that curvature of sporozoite tracks engaging with vasculature optimizes contact with dermal capillaries. Imaging of sporozoites with mutations in key adhesive proteins highlight the importance of the sporozoite's gliding speed and its ability to modulate adhesive properties for successful exit from the inoculation site.
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Base de dados:
MEDLINE
Assunto principal:
Plasmodium
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Vasos Sanguíneos
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Derme
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Esporozoítos
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Locomoção
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Malária
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article