Detection of the F129L mutation in the cytochrome b gene in Phakopsora pachyrhizi.
Pest Manag Sci
; 72(6): 1211-5, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-26296393
ABSTRACT
BACKGROUND:
Asian soybean rust, caused by Phakopsora pachyrhizi, is mostly controlled by demethylation inhibitor (DMI) and quinone outside inhibitor (QoI) fungicides. Mutations in the cytochrome b (CYTB) gene can lead to pathogen resistance to QoIs. The occurrence of the mutations in codons 129, 137 and 143 in the CYTB gene was investigated, and a pyrosequencing assay was developed for rapid and quantitative detection of the F129L mutation.RESULTS:
Molecular analysis of the CYTB gene showed the presence of the F129L mutation in field samples and monouredinial isolates, while other mutations (G143A and G137R) were not found. The pyrosequencing was an effective method for quantitative detection of the F129L mutation, and many of the P. pachyrhizi samples showed high frequency of F129L.CONCLUSION:
This is the first report of the occurrence of the F129L mutation in P. pachyrhizi. The practical relevance of this mutation for field efficacy of QoIs needs further investigation. © 2015 Society of Chemical Industry.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Farmacorresistência Fúngica
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Citocromos b
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Phakopsora pachyrhizi
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article