Determination of tocopherols and sitosterols in seeds and nuts by QuEChERS-liquid chromatography.
Food Chem
; 192: 825-30, 2016 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-26304416
ABSTRACT
In the present work a simple, reliable and affordable sample treatment method for the simultaneous analysis of tocopherols and free phytosterols in nuts was developed. Analyte extraction was carried out using the QuEChERS methodology and analyte separation and detection were accomplished using HPLC-DAD. The use of this methodology for the extraction of natural occurring substances provides advantages such as speed, simplicity and ease of use. The parameters evaluated for the validation of the method developed included the linearity of the calibration plots, the detection and quantification limits, repeatability, reproducibility and recovery. The proposed method was successfully applied to the analysis of tocopherols and free phytosterols in samples of almonds, cashew nuts, hazelnuts, peanuts, tiger nuts, sun flower seeds and pistachios.
Palavras-chave
Alpha-tocopherol (PubChem CID: 2116); Beta-sitosterol (PubChem CID: 222284); Brassicasterol (PubChem CID: 5281327); Campesterol (PubChem CID: 173183); Delta-tocopherol (PubChem CID: 92094); Gamma-tocopherol (PubChem CID: 92729); LC; Nuts; Phytosterols; QuEChERS; Stigmasterol (PubChem CID: 5280794); Tocopherols
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sementes
/
Sitosteroides
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Cromatografia Líquida
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Tocoferóis
/
Nozes
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article