α-Dendrotoxin inhibits the ASIC current in dorsal root ganglion neurons from rat.
Neurosci Lett
; 606: 42-7, 2015 Oct 08.
Article
em En
| MEDLINE
| ID: mdl-26314509
ABSTRACT
Dendrotoxins are a group of peptide toxins purified from the venom of several mamba snakes. α-Dendrotoxin (α-DTx, from the Eastern green mamba Dendroaspis angusticeps) is a well-known blocker of voltage-gated K(+) channels and specifically of K(v)1.1, K(v)1.2 and K(v)1.6. In this work we show that α-DTx inhibited the ASIC currents in DRG neurons (IC50=0.8 µM) when continuously perfused during 25 s (including a 5 s pulse to pH 6.1), but not when co-applied with the pH drop. Additionally, we show that α-DTx abolished a transient component of the outward current that, in some experiments, appeared immediately after the end of the acid pulse. Our data indicate that α-DTx inhibits ASICs in the high nM range while some Kv are inhibited in the low nM range. The α-DTx selectivity and its potential interaction with ASICs should be taken in consideration when DTx is used in the high nM range.
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MEDLINE
Assunto principal:
Bloqueadores dos Canais de Potássio
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Venenos Elapídicos
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Canais Iônicos Sensíveis a Ácido
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Gânglios Espinais
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Neurônios
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article