Inntags: small self-structured epitopes for innocuous protein tagging.
Nat Methods
; 12(10): 955-8, 2015 Oct.
Article
em En
| MEDLINE
| ID: mdl-26322837
ABSTRACT
Protein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Imunofluorescência
/
Imunoprecipitação
/
Epitopos
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article