Rapid detection of genetically diverse tomato black ring virus isolates using reverse transcription loop-mediated isothermal amplification.
Arch Virol
; 160(12): 3075-8, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26338092
ABSTRACT
A reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) has been developed for detection of tomato black ring virus (TBRV) isolates collected from different hosts. One-step RT-LAMP was performed with a set of four primers, the design of which was based on the coat protein gene. Results of RT-LAMP were visualized by direct staining of products with fluorescent dyes, agarose gel electrophoresis, and analysis of amplification curves. The sensitivity of RT-LAMP was 100-fold greater than that of RT-PCR. The RT-LAMP assay developed here is a useful and practical method for diagnosis of TBRV.
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Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
/
Nepovirus
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Solanum lycopersicum
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Técnicas de Amplificação de Ácido Nucleico
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article