LL-37 immunomodulatory activity during Mycobacterium tuberculosis infection in macrophages.
Infect Immun
; 83(12): 4495-503, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26351280
ABSTRACT
Tuberculosis is one of the most important infectious diseases worldwide. The susceptibility to this disease depends to a great extent on the innate immune response against mycobacteria. Host defense peptides (HDP) are one of the first barriers to counteract infection. Cathelicidin (LL-37) is an HDP that has many immunomodulatory effects besides its weak antimicrobial activity. Despite advances in the study of the innate immune response in tuberculosis, the immunological role of LL-37 during M. tuberculosis infection has not been clarified. Monocyte-derived macrophages were infected with M. tuberculosis strain H37Rv and then treated with 1, 5, or 15 µg/ml of exogenous LL-37 for 4, 8, and 24 h. Exogenous LL-37 decreased tumor necrosis factor alpha (TNF-α) and interleukin-17 (IL-17) while inducing anti-inflammatory IL-10 and transforming growth factor ß (TGF-ß) production. Interestingly, the decreased production of anti-inflammatory cytokines did not reduce antimycobacterial activity. These results are consistent with the concept that LL-37 can modulate the expression of cytokines during mycobacterial infection and this activity was independent of the P2X7 receptor. Thus, LL-37 modulates the response of macrophages during infection, controlling the expression of proinflammatory and anti-inflammatory cytokines.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos Catiônicos Antimicrobianos
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Fatores Imunológicos
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Macrófagos
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Mycobacterium tuberculosis
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article