Association of HADHA with human RNA silencing machinery.
Biochem Biophys Res Commun
; 466(3): 481-5, 2015 Oct 23.
Article
em En
| MEDLINE
| ID: mdl-26367179
ABSTRACT
In RNA silencing, small RNAs produced by dicer mediate target repression guided by RNA induced silencing complex (RISC). To effectively mediate this response, one or more proteins are employed at each stage. In the present study, we investigated HADHA, a new component in the human RNA silencing machinery. Immunoprecipitation analysis revealed that, HADHA associates with dicer complex and immunohistochemical studies confirmed its co localization with Dicer in the cytoplasm. Further, over expression of HADHA resulted in higher abundance levels of mature miRNA against a reduction in respective precursor levels and vice versa in HADHA knocked down cells. These findings suggest an auxiliary role for HADHA in miRNA biogenesis and help in better understanding of molecular mechanisms underlying RNAi pathway in mammals.
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Base de dados:
MEDLINE
Assunto principal:
Complexo de Inativação Induzido por RNA
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Interferência de RNA
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Subunidade alfa da Proteína Mitocondrial Trifuncional
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article