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Concept and design of a genome-wide association genotyping array tailored for transplantation-specific studies.
Li, Yun R; van Setten, Jessica; Verma, Shefali S; Lu, Yontao; Holmes, Michael V; Gao, Hui; Lek, Monkol; Nair, Nikhil; Chandrupatla, Hareesh; Chang, Baoli; Karczewski, Konrad J; Wong, Chanel; Mohebnasab, Maede; Mukhtar, Eyas; Phillips, Randy; Tragante, Vinicius; Hou, Cuiping; Steel, Laura; Lee, Takesha; Garifallou, James; Guettouche, Toumy; Cao, Hongzhi; Guan, Weihua; Himes, Aubree; van Houten, Jacob; Pasquier, Andrew; Yu, Reina; Carrigan, Elena; Miller, Michael B; Schladt, David; Akdere, Abdullah; Gonzalez, Ana; Llyod, Kelsey M; McGinn, Daniel; Gangasani, Abhinav; Michaud, Zach; Colasacco, Abigail; Snyder, James; Thomas, Kelly; Wang, Tiancheng; Wu, Baolin; Alzahrani, Alhusain J; Al-Ali, Amein K; Al-Muhanna, Fahad A; Al-Rubaish, Abdullah M; Al-Mueilo, Samir; Monos, Dimitri S; Murphy, Barbara; Olthoff, Kim M; Wijmenga, Cisca.
Afiliação
  • Li YR; Medical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • van Setten J; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Verma SS; Department of Cardiology, Division of Heart and Lungs, University Medical Center Utrecht, Utrecht, The Netherlands.
  • Lu Y; Center for Systems Genomics, The Pennsylvania State University, University Park, PA, USA.
  • Holmes MV; Affymetrix Incorporated, Santa Clara, CA, USA.
  • Gao H; Penn Transplant Institute, Hospital of the University of Pennsylvania, Philadelphia, PA, USA.
  • Lek M; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Nair N; Penn Transplant Institute, Hospital of the University of Pennsylvania, Philadelphia, PA, USA.
  • Chandrupatla H; Analytic and Translational Genetics Unit, Massachusetts General Hospital, Boston, MA, USA.
  • Chang B; Program in Medical and Population Genetics, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
  • Karczewski KJ; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Wong C; Penn Transplant Institute, Hospital of the University of Pennsylvania, Philadelphia, PA, USA.
  • Mohebnasab M; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Mukhtar E; Penn Transplant Institute, Hospital of the University of Pennsylvania, Philadelphia, PA, USA.
  • Phillips R; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Tragante V; Penn Transplant Institute, Hospital of the University of Pennsylvania, Philadelphia, PA, USA.
  • Hou C; Analytic and Translational Genetics Unit, Massachusetts General Hospital, Boston, MA, USA.
  • Steel L; Program in Medical and Population Genetics, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
  • Lee T; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Garifallou J; Penn Transplant Institute, Hospital of the University of Pennsylvania, Philadelphia, PA, USA.
  • Guettouche T; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Cao H; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Guan W; Penn Transplant Institute, Hospital of the University of Pennsylvania, Philadelphia, PA, USA.
  • Himes A; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • van Houten J; Penn Transplant Institute, Hospital of the University of Pennsylvania, Philadelphia, PA, USA.
  • Pasquier A; Department of Cardiology, Division of Heart and Lungs, University Medical Center Utrecht, Utrecht, The Netherlands.
  • Yu R; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Carrigan E; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Miller MB; Penn Transplant Institute, Hospital of the University of Pennsylvania, Philadelphia, PA, USA.
  • Schladt D; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Akdere A; Penn Transplant Institute, Hospital of the University of Pennsylvania, Philadelphia, PA, USA.
  • Gonzalez A; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Llyod KM; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • McGinn D; BGI-Shenzhen, Shenzhen, China.
  • Gangasani A; Department of Biology, University of Copenhagen, Copenhagen, Denmark.
  • Michaud Z; Division of Biostatistics, University of Minnesota, Minneapolis, MN, USA.
  • Colasacco A; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Snyder J; Penn Transplant Institute, Hospital of the University of Pennsylvania, Philadelphia, PA, USA.
  • Thomas K; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Wang T; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Wu B; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Alzahrani AJ; The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Al-Ali AK; Department of Psychology, University of Minnesota, Minneapolis, MN, USA.
  • Al-Muhanna FA; Minneapolis Medical Research Foundation, Hennepin County Medical Center, Minneapolis, MN, USA.
  • Al-Rubaish AM; Medical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Al-Mueilo S; Medical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Monos DS; Medical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Murphy B; Medical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Olthoff KM; Medical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Wijmenga C; Medical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Genome Med ; 7: 90, 2015 Oct 01.
Article em En | MEDLINE | ID: mdl-26423053
ABSTRACT

BACKGROUND:

In addition to HLA genetic incompatibility, non-HLA difference between donor and recipients of transplantation leading to allograft rejection are now becoming evident. We aimed to create a unique genome-wide platform to facilitate genomic research studies in transplant-related studies. We designed a genome-wide genotyping tool based on the most recent human genomic reference datasets, and included customization for known and potentially relevant metabolic and pharmacological loci relevant to transplantation.

METHODS:

We describe here the design and implementation of a customized genome-wide genotyping array, the 'TxArray', comprising approximately 782,000 markers with tailored content for deeper capture of variants across HLA, KIR, pharmacogenomic, and metabolic loci important in transplantation. To test concordance and genotyping quality, we genotyped 85 HapMap samples on the array, including eight trios.

RESULTS:

We show low Mendelian error rates and high concordance rates for HapMap samples (average parent-parent-child heritability of 0.997, and concordance of 0.996). We performed genotype imputation across autosomal regions, masking directly genotyped SNPs to assess imputation accuracy and report an accuracy of >0.962 for directly genotyped SNPs. We demonstrate much higher capture of the natural killer cell immunoglobulin-like receptor (KIR) region versus comparable platforms. Overall, we show that the genotyping quality and coverage of the TxArray is very high when compared to reference samples and to other genome-wide genotyping platforms.

CONCLUSIONS:

We have designed a comprehensive genome-wide genotyping tool which enables accurate association testing and imputation of ungenotyped SNPs, facilitating powerful and cost-effective large-scale genotyping of transplant-related studies.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Estudo de Associação Genômica Ampla / Genótipo Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Estudo de Associação Genômica Ampla / Genótipo Idioma: En Ano de publicação: 2015 Tipo de documento: Article