Cre Recombinase: You Can't Live with It, and You Can't Live Without It.
Adv Exp Med Biol
; 854: 725-30, 2016.
Article
em En
| MEDLINE
| ID: mdl-26427481
ABSTRACT
The development of conditional gene targeting has greatly advanced our knowledge of human retinal diseases, but issues have arisen related to the use of some Cre-expressing mouse lines. In this article, we discuss potential problems associated with transgenic Cre expression-induced degeneration and alteration of rod photoreceptors and retinal pigment epithelium (RPE). Our strategy for circumventing RPE degeneration by induced transient Cre expression uses a single intravitreal doxycycline injection in a tetracycline-inducible RPE-specific Cre mouse line, which results in productive Cre-mediated recombination efficiently in the RPE. As constitutive expression of Cre in the RPE alters RPE biology, this inducible Cre/lox system provides an opportunity for conditional gene targeting in the RPE, a tissue that is closely related to photoreceptor degeneration, age-related macular degeneration, and diabetic retinopathy.
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Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
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Degeneração Retiniana
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Marcação de Genes
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Integrases
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article