Purification and characterization of saxitoxin from Mytilus chilensis of southern Chile.
Toxicon
; 108: 147-53, 2015 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-26435338
ABSTRACT
In the current communication we describe an innovative method to purify saxitoxin (STX), a toxin presents in contaminated muscle of Mylitus chilensis extracted in the southern part of Chile, using a liquid chromatographic methodology based on ionic pairs. The STX was extracted using HCl and treated with ammonium sulfate following a treatment with trichloroacetic acid and hexane/diethyl ether (97/3). The samples were analyzed by a semi-preparative HPLC in order to collect pure fractions of STX and these fractions were eluted in solid-phase cationic interchange SCX extraction columns. The purified STX was stable and homogeneous and its identity was confirmed by LC-MS-MS, which demonstrated a high quality purification of STX, without presence of analogs such as neosaxitoxin (Neo) and decarbamoyl saxitoxin (dcSTX). The STX biological activity was analyzed in a bioassay in mice model and compared to the standard STX produced by the FDA and no significant differences were observed.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Saxitoxina
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Cromatografia Líquida de Alta Pressão
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Mytilus
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article