Inhibition of microRNA-9-3p reduces lipid accumulation in HepG2 cells by targeting the expression of sirtuin type 1.
Mol Med Rep
; 12(5): 7742-8, 2015 Nov.
Article
em En
| MEDLINE
| ID: mdl-26459099
ABSTRACT
Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder caused by the interaction of environmental factors and multiple genes. The genetic background of T2DM is complex and remains to be fully elucidated. MicroRNAs (miRNAs) are negative regulators of gene expression and several miRNAs are associated with the development of T2DM. However, the expression and biological function of miRNA93p in lipid metabolism of patients with T2DM remain to be fully elucidated. The predominant aim of the present study was to examine the effect of miRNA93p on lipid accumulation in HepG2 cells. To investigate this, an MTT assay was used to determine cell proliferation, and the effects of miRNA93p on triglycerides (TG) and total cholesterol (TC) in the HepG2 cells were also examined. Reverse transcriptionquantitative polymerase chain reaction and western blot analyses were used to measure the expression levels of SIRT1 at the gene and protein levels, respectively. The date revealed that downregulation of miRNA93p inhibited the proliferation of HepG2 cells, and significantly reduced the accumulation of lipids, and decreased TG and TC content. In addition, the present study demonstrated that inhibition of miRNA93p increased the protein expression of sirtuin type 1 (SIRT1), but had no effects on the gene expression of SIRT1. Therefore, these findings demonstrated that the inhibition of miRNA93p reduced the proliferation of HepG2 cells and lipid accumulation by upregulating the expression of SIRT1, indicating its potential as a therapeutic target.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
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Diabetes Mellitus Tipo 2
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Metabolismo dos Lipídeos
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Células Hep G2
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Sirtuína 1
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article