Complementation analysis reveals a potential role of human ARV1 in GPI anchor biosynthesis.

Ikeda, Atsuko; Kajiwara, Kentaro; Iwamoto, Kunihiko; Makino, Asami; Kobayashi, Toshihide; Mizuta, Keiko; Funato, Kouichi

Ikeda, Atsuko; Kajiwara, Kentaro; Iwamoto, Kunihiko; Makino, Asami; Kobayashi, Toshihide; Mizuta, Keiko; Funato, Kouichi.

Afiliação

Ikeda A; Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
Kajiwara K; Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
Iwamoto K; Lipid Biology Laboratory and RIKEN, Saitama, Japan.
Makino A; Lipid Biology Laboratory and RIKEN, Saitama, Japan.
Kobayashi T; Lipid Biology Laboratory and RIKEN, Saitama, Japan.
Mizuta K; Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
Funato K; Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.

Yeast ; 33(2): 37-42, 2016 Feb.

Article em En | MEDLINE | ID: mdl-26460143

ABSTRACT

ABSTRACT

ARV1 is involved in regulating lipid homeostasis but also in the biosynthesis of glycosylphosphatidylinositol (GPI) in Saccharomyces cerevisiae. Here, we examined whether human ARV1 can complement the role of yeast ARV1 in GPI biosynthesis. Overexpression of human ARV1 could rescue the phenotypes associated with GPI anchor synthesis defect in the yeast arv1Δ mutant. The results suggest that Arv1 function in GPI biosynthesis may be conserved in all eukaryotes, from yeast to humans.

Assuntos

Proteínas de Transporte/metabolismo; Glicosilfosfatidilinositóis/biossíntese; Proteínas de Membrana/metabolismo; Proteínas de Saccharomyces cerevisiae/metabolismo; Proteínas de Transporte/genética; Expressão Gênica; Teste de Complementação Genética; Homeostase; Humanos; Metabolismo dos Lipídeos; Proteínas de Membrana/genética; Mutação; Saccharomyces cerevisiae/genética; Saccharomyces cerevisiae/metabolismo; Proteínas de Saccharomyces cerevisiae/genética

Palavras-chave

GPI anchor; arv1Δ mutant; complementation; human ARV1; yeast

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Transporte / Glicosilfosfatidilinositóis / Proteínas de Saccharomyces cerevisiae / Proteínas de Membrana Idioma: En Ano de publicação: 2016 Tipo de documento: Article

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